Professor Matthew R. Pratt Professor Chao Zhang Department of Chemistry Last updated September 17, 2018 Laboratory Assignment #3:

Professor Matthew R. Pratt Professor Chao Zhang Department of Chemistry

Last updated September 17, 2018

Haven’t Found The Relevant Content? Hire a Subject Expert to Help You With
Professor Matthew R. Pratt Professor Chao Zhang Department of Chemistry Last updated September 17, 2018 Laboratory Assignment #3:
Post Your Own Question And Get A Custom Answer
Hire Writer

Laboratory Assignment #3: Protein Database

Remember that each student needs to turn in their own work in their own words, even if you work together! To ensure proper ownership of ideas, you must list the full name(s) of your classmate(s) that you worked with in the appropriate field. You can use a combination of the laboratory exercise, the internet, and the AIDS Update book to answer the following questions, but be aware that your answers will be checked for plagiarism. Be sure to give references for your answers.

The HIV virus is an assembly of both proteins (viral enzymes, matrix and envelope proteins) and RNA genes. In addition to what you learned in class about proteins, mature proteins can be made up of several different chains of amino acids. If a protein is made up of only one chain, like the ones we saw in class, they are called monomers. However, if they are made of two chains, they are called dimers, and so on. The viral proteins, and possibly the genes themselves, are targets for drug design. We will learn about HIV-1 protease and the inhibitors used against it in class. In this lab, we will study the 3D structure of another HIV enzyme, HIV-1 reverse transcriptase, and examine how the structure may be used for rational drug design. You can use a combination of the laboratory exercise, the internet, and the AIDS Update book to answer the following questions, but be aware that your answers will be checked for plagiarism.

1. (5 points) HIV-1 Reverse Transcriptase Background.

What is the function of HIV-1 reverse transcriptase? Specifically, what does HIV-1 reverse transcriptase do in a biochemical sense?

2. (5 points) HIV-1 Reverse Transcriptase Structure. Using the applet supplied in this lab in the lab data link, look at the structure of HIV-1 reverse transcriptase.

(a) Is the enzyme a monomer (1 chain), a dimer (2 chains), a trimer (3 chains), etc.?

(b) Which chain is composed of greatest number of amino acids?

(c) Which chain has the greater number of alpha helix segments? (d) What is the most predominant amino acid in each chain?

3. (4 points) Reverse Transcriptase Inhibitors.

Using the applet supplied in this lab in the lab data link, look at the structure of the

HIV-1 reverse transcriptase inhibitor.

(a) Select the miscellaneous molecule (inhibitor), display it in atom and bonds format, save it as a jpg and upload it into your lab report without the enzyme subunits.

(b) How many atoms does it possess (excluding hydrogen atoms as shown)?

4. (6 points) What property of HIV-1 reverse transcriptase makes it difficult to use single drugs that remain effective in treating infection? Hint: It is a property that our DNA and RNA polymerases have evolved to avoid. 

My Homework Geeks

Why Hire a Professional Essay Writer from MyHomeworkGeeks

Quality Urgent Essays

Domyhomeworkk online platform provides the best essay writing service ever received elsewhere, thanks to our professional essay writers. Experience gained in the 10+ years of being in the assignment writing industry is also an added advantage.

Professional and Experienced Academic Writers

A lot is done to maintain us as the best essay writing service provider. Besides owning a degree from a recognized university, a writer must pass the rigorous tests we take them through before they are considered eligible to offer urgent essay help on our website.

Affordable Urgent Assignment Help

Who said a reliable website should not be cheap also? While it is human nature to associate prices with quality, that does not always work. At domyhomeworkk.com, we guarantee you cheap and urgent essay help without compromising the quality.

24/7 Available Urgent Essay Writing Service

Whether it is a ‘write my essay for me cheap’ or ‘edit my essay’ order you want to request, always feel free to reach us at any time of the day. Our experts work in shifts to ensure that you access urgent essay writing services at any time of the day.

Plagiarism Free Essays Online

The punitive measures that come with plagiarized content are so harsh, and we understand that. We consequently ensure to write each client’s papers from scratch no matter how urgent the essay is. To acknowledge borrowed content, our quick writer references and cites the work.

Wow Customer Support 24/7

Our support agents are available 24 hours a day 7 days a week and committed to providing you with the best customer experience. Get in touch whenever you need any assistance.

Try it now!

Calculate the price of your order

Total price:
$0.00

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

So much stress and so little time? Take care of yourself: let us help you with your tasks. We offer all kinds of writing services.

Essays

Custom Essay Writing Services

No matter what kind of academic paper you need and how urgent you need it, you are welcome to choose your academic level and the type of your paper at an affordable price. We take care of all your paper needs and give a 24/7 customer care support system.

Admissions

Admission and Business Papers

An admission essay is an essay or other written statement by a candidate, often a potential student enrolling in a college, university, or graduate school. You can be rest assurred that through our service we will write the best admission essay for you.

Editing

Editing and Proofreading

Our academic writers and editors make the necessary changes to your paper so that it is polished. We also format your document by correctly quoting the sources and creating reference lists in the formats APA, Harvard, MLA, Chicago / Turabian.

Coursework

Revision Support

If you think your paper could be improved, you can request a review. In this case, your paper will be checked by the writer or assigned to an editor. You can use this option as many times as you see fit. This is free because we want you to be completely satisfied with the service offered.

Have a homework question? Get help from verified tutors now!